Summary of Interesting Results Here are some of the newest, most interesting results of the study: tbd

Ampulloclitocybe - click to expand Clitocyboid. Grey or brown slightly umbonate (yet depressed in age) caps, strongly decurrent white gills. Resembles Cantharellula (below) but without the forking gills that stain red in age and without amyloid spores. (Pseudoclitocybe in the Tricholomatineae has no umbo, no forking, no staining and amyloid spores). Lacking amyloid spores, this genus is especially difficult to separate from Clitocybe. If the umbo is obvious, that will help. Species mentioned: Ampulloclitocybe clavipes, avellaneialba, avellaneoalba. Clitocybe subclavipes.   Ampulloclitocybe 'clavipes PNW01' - grey or brown cap with a small umbo (yet depressed in age), strongly decurrent white gills, a club stem and often a grape bubble gum odor when fresh (but sometimes not). Bigelow reported Clitocybe clavipes EU (now Ampulloclitocybe clavipes) as very common here, and the odorless, slightly microscopically different Clitocybe subclavipes NY (nobody has bothered to move it to Ampulloclitocybe yet) as being here as well. He noted that out west there was less of a bulb on the stem because the stems were overall thicker to begin with. Well, that character is significant, as our species is likely undescribed, 2% different in ITS. The two named species may be distinct, although only 3 bp and 2 indels separate them, but neither of them have been proven to be here. Our species needs to be described with a thicker stem (making the bulb less pronounced) and that it may or may not have the odor at easily detectable levels. Ampulloclitocybe sub-sub-clavipes anyone? Ampulloclitocybe avellaneialba WA - (formerly misspelled A. avellaneoalba), usually with a darker grey or brown cap that may have a slightly ribbed cap margin. Lacking the bulb and the odor. This species may occur on or near rotting wood and can get quite large. Don't mistake them for the thinner-fleshed Gerronema atrialbum. Not always darker than A. 'clavipes PNW01', as you can see, the darker of the two A. 'clavipes PNW01' photos is much like the paler of the two A. avellaneialba photos. Then you have to consider the amount of odor and stem bulb. Ampulloclitocybe 'clavipes PNW01' © Yi-Min Wang, NAMA and the Field Museum of Natural History,     A. avellaneialba © Yi-Min Wang and Yi-Min Wang

Arrhenia s.l. - click to expand There are two very distinct looks for Arrhenia, and some species of the first type don't have strong support for belonging to this genus. small brown to dark brown (rarely greenish) omphalinoids on wood or moss (other omphalinoids are not usually as dark brown, but consider Omphalina itself for yellow-brown, warm brown, or pinkish-red brown species on moss, and Lichenomphalia below if the stem base has granular or leafy lichen mateial). small dark brown (rarely pale grey) "oysters" with eccentric/lateral/missing stems (but growing on the ground in moss, not on wood) sometimes with poorly developed gills. Rimbachia and Muscinupta, both usually pure white (unlike Arrhenia), also may lack gills and grow on moss on the ground. One possible interpretation of Rimbachia (genus B) is near Arrhenia and covered here. Muscinupta has a tapering fluted "stem". Species mentioned: Arrhenia acerosa, subglobisemen, lobata, auriscalpium, retiruga, chlorocyanea, epichysium, hohensis, latispora, obscurata, onisca, peltigerina, pubescentipes, rainierensis, sphagnicola. Rimbachia neckerae.   My ITS only tree shows that Arrhenia, as well as Lichenomphalia below, fall into 2 distinct clades far enough from each other and it appears that a few of the species might need to be moved to their own genus. In this case, a few of the omphalinoid Arrhenias appear to be only distantly related to the others. I would like a multi-gene study to verify this, as mine is not the only study to show that all Arrhenias don't necessarily belong together. All oyster-like Arrhenias belong in Arrhenia proper. Rimbachia B is a genus separate from both. Dictyonema basidiolichens seem paraphyletic inside Arrhenia, giving more evidence that Arrhenia needs to be split.   1. Moss associates with eccentric stems and possibly poorly developed gills These species don't appear to all group together (they may have gained or lost this shape in more than one event) so we probably can't give them their own genus. We just have to accept that there are two very distinct looks for Arrhenia. Similar looking mushrooms are found in Rimbachia (Tricholomatineae) and Muscinupta (Hymenochaetales), but those are pure white. Arrhenia 'subglobisemen PNW06' - brown, short lateral stem, fairly well developed gills. It was reported that we have Arrhenia acerosa EU here, but three sequences turn out to be 2% different than A. subglobisemen instead and much further away from A. acerosa. If anybody thinks A. acerosa is really here, save a collection because we have EU DNA to compare to. It is a complex where ITS DNA can vary by 2%, and has spores that vary widely in shape, often being almost round like in A. subglobisemen, so that may have created the confusion, and if the spore shape corresponds with certain ITS sequences in the complex, it could also mean A. acerosa will be split up into more than one species. Arrhenia cf. lobata EU - brown, stemless, poorly developed wrinkle-like "gills". Reported from the PNW, and we have EU DNA to compare to, but no collections to prove it's really here. We need some, especially since it's similar to the previous species. Arrhenia 'auriscalpium PNW07' - long lateral stem, slightly veined underside. One North American sequence is 2% different than type area EU sequences. We don't have local sequences (we need them) but ours is probably a sister species and not the real thing. Arrhenia 'retiruga PNW08' - paler tan or grey felty cap, stemless, almost smooth underneath. One WA sequence is 3% different than 2 competing concepts in the EU for what this species is. Ours likely needs a new name. Arrhenia 'retiruga PNW12' - another species in this complex, found once in Idaho. Arrhenia PNW14 - found on a mossy oak log, resembles A. retiruga, but isn't in the same clade as that and PNW08 and PNW12. Rimbachia B neckerae EU - a tiny white cup fungus a few mm across on moss, without gills and attached from the centre of what would be the top of the cap. Elliptical spores (unlike the round spored Rimbachia A arachnoidea NY). R. neckerae was reported once from the PNW. Rimbachia B is in a genus near Arrhenia. We won't know which is the real genus, A or B, until we sequence the type of the genus, Rimbachia paradoxa EU. We have one possible EU sequence of R. neckerae, but we have no local sequences so we need collections. Rimbachia A belongs in the Omphalinaceae and the following two species are discussed there: Rimbachia A arachnoidea NY - a tiny white cup fungus a few mm across on moss, without gills and attached from the centre of what would be the top of the cap. Round spores. We have EU sequences, but no ENA type area sequences to know for sure what this species is. Nor do we have any local sequences, we need collections. Rimbachia A bryophila EU - a tiny white stemless oyster a few mm across, with rudimentary gills, growing on moss, not wood. We have three matching CA sequences and an OR sequence, but no EU sequences to compare with to make sure ours is the same species. Arrhenia 'subglobisemen PNW06' © Matthew Koons,     A. 'auriscalpium PNW07' © Ben Woo,     A. 'retiruga PNW08' © NAMA and the Field Museum of Natural History,     A. 'retiruga PNW12' © Ed Barge,     A. PNW14 © Michael Beug   2. Brown (rarely green) omphalinoid mushrooms 'Clitocybe' atroviridis WA (=Arrhenia chlorocyanea EU?) - This unique, beautiful dark green omphalinoid, found on the ground often in moss, was described from WA as Clitocybe atroviridis but that is thought to be a synonym of the older EU species Arrhenia chlorocyanea. We don't have any EU sequences of that mushroom to compare to, and given that our local species of other Arrhenias has turned out to be different than the EU counterpart that was thought to be the same, we need EU sequences to find out for sure. If not, we'll need to rename ours to Arrhenia atroviridis. unsequenced Arrhenia chlorocyanea © Ben Woo   'Arrhenia2'  telmatiaea UK (='Omphalina' fusconigra UK) - recognizable by its dark brown smooth cap and growth in sphagnum. It is in the "other" clade. Two OR sequences match the type sequence of this newly described species. One theory is that Bigelow's reports of Arrhenia onisca, which could not have been that species as it is a synonym of Lichenomphalia ericetorum, represent A. telmatiaea. 'Arrhenia2' philonotis EU - a paler brown smooth cap and growth in sphagnum. Also in the "other" clade. Two OR sequences match the type sequence. 'Arrhenia2' velutipes UK - one WA collection of a dark species in polytrichum moss turned out to match this species. 'Arrhenia2' telmatiaea and 'Arrhenia2' philonotus © Connor Dooley,     'Arrhenia2' velutipes © Graham Steinruck   'Arrhenia2' bigelowii MI (=Arrhenia gerardiana var. fusca NY) - recognized by having a scaly cap in sphagnum moss. One WA collection matches the type sequence. Formerly, it was thought that this species/variety always had a darker cap than Arrhenia gerardiana, but our local collection confirms this is not the case. Dark capped collections are A. bigelowii. Not-so-dark capped collections could be either A. bigelowii or A. gerardiana (indistuingishable so far except for by DNA, as the supposed different sized spores of A. gerardiana doesn't seem to hold up). Therefore, local reports of A. gerardiana need confirmation as it is not yet confirmed by DNA from the PNW. This is in the "other" clade of Arrhenia. 'Arrhenia2' bigelowii © Sadie Hickey (2 images)   Arrhenia 'latispora PNW09' - a moss species (not just sphagnum?) with wide spores (7.8-9.5 x 6.5u) and said to have a geranium odor at least in the EU, where it was described. 2 collections from OR and 1 from WA match the EU epitype sequence closely except for a couple locations in ITS2, however that species is described as a pleurotoid oyster-like mushroom, very different than what ours looks like, so I believe it is unique. The same thing is happening with Arrhenia TN01 and A. acerosa. Arrhenia 'obscurata PNW11' - Arrhenia obscurata UK (=Omphalina atrobrunnea UK) is found on sandy soil or moss, reported from ID and BC. We don't have any UK sequences, but Voitk provides some ENA sequences. Our one WA collection is at least 2% different in ITS. It's possible ours will match true UK sequences of A. obscurata, but until we prove that, I'm giving it a code. Arrhenia TN01 - very dark in soil with moss. This is in a clade of species mostly labeled Arrhenia acerosa, a pleurotoid with rudimentary gills and a lateral stem. However, this species has normal gills and a central stem. This unexpected shape difference also occurs with A. 'latispora PNW09' above. Our local pleurotoid species, Arrhenia 'subglobisemen PNW06' is not even in this clade. Arrhenia 'latispora PNW09' © Connor Dooley and Matthew Koons,     Arrhenia 'obscurata PNW11' © Matthew Koons,     A. TN01 © Connor Dooley   Arrhenia 'peltigera PNW15' - on Peltigera lichen. This is a complex of species in its type area of NY. Bigelow claims to have found this in Idaho. The one collection we sequenced, from WA, was not any of the east coast concepts and may need its own name. Arrhenia 'peltigera PNW15' © Matthew Koons   The following lookalike dark brown omphalinoid species, from the ground or moss, are reported from here but without sequenced, photographed collections yet. 'Arrhenia onisca' EU - Bigelow reported this from the PNW on moss or burned moss, but it's now known that this name is a junior synonym of Lichenomphalia ericetorum, so nobody knows what Bigelow really found. My leading theory is Arrhenia telmatiaea, described above. 'Arrhenia sphagnicola' EU - This name too has officially been declared a synonym of Lichenomphalia ericetorum, so it is no longer being used. This name has been used for reports of A. bigelowii and A. telmatiaea back when A. sphagnicola was incorrectly thought to be a synonym of Omphalina gerardiana and O. fusconigra, but this name should not be used anymore. Arrhenia rainierensis WA - on soil or moss with interesting shaped spores. Described from here but we don't have any reliable sequences. I don't even know which clade it's in. Arrhenia pubescentipes ID - on soil or moss. No local sequences, so no idea which clade it's in. 'Arrhenia2' hohensis WA - on soil or moss. Described from here but we have no sequences. One mycologist suggested it might be similar to or the same as the EU species Arrhenia obatra, if so, that species appears to be one of those possibly needing to be moved to the new genus, so maybe A. hohensis does too, but maybe not.   These two local species below are in the true Arrhenia clade. Arrhenia PNW01 - 1 collection from OR Arrhenia PNW10 - 2 collections from WA, in Polytrichum moss, with a somewhat greasy cap with a wavy margin.   Arrhenia PNW01 OR © Bitty Roy,     Arrhenia PNW10 © iNaturalist user fish_narc (2 images)    These species are in the "other" clade. 'Arrhenia2' 'epichysium PNW03' - a very dark, terrestrial species not with moss, in a clade with the other A. epichysium species. Found 3 times in WA so far. Bigelow found it in Idaho and mistook it for A. peltigera. 'Arrhenia2' 'epichysium CA01' - very dark, terrestrial, on non-sphagnum moss. Found once in WA. It is close to one possible concept of the EU species A. epichysium, which is found on wood. 'Arrhenia2' 'epichysium CA02/NB01' - very dark when fresh (drying pale), on wood, close to the other possible concept of A. epichysium. The habitat on wood matches the true A. epichysium. West coast sequences (CA02) and east coast sequences (NB01) are almost the same, but WA/CA collections were darker than ENA collections. Then one AK collection was quite hygrophanous and showed it probably starts out dark and gets quite pale on drying out. Although there are competing concepts in the EU for what 'Arrhenia' epichysium is, none of them are found here, as has been reported. Those reports may be one of the above three species.   'Arrhenia2' 'epichysium PNW03' © Yi-Min Wang,     'Arrhenia2' 'epichysium CA01' © Larry Martin,     'Arrhenia2' 'epichysium CA02' © Jack Johnson   'Arrhenia2' PNW04 - 3 collections from WA. It may have a scaly cap and may develop marginate gill edges. 'Arrhenia2' PNW05 - on soil, with highly contrasting colour striations on the cap. 1 collection from OR. 'Arrhenia2' PNW13 - on moss, small and also very contrastingly striate. This matches some (but not all) EU sequences of Arrhenia obatra EU, and looks like it could be Arrhenia hohensis WA, which may or may not be a synonym. We need some type sequences to verify what this is. 'Arrhenia2' PNW04 © Yi-Min Wang and Matthew Koons,     'Arrhenia2' PNW05 © Connor Dooley,     'Arrhenia2' PNW13 © Lauren Ré (2 images)

Cantharellula - click to expand Clitocyboid. Grey or brown slightly umbonate (yet depressed in age) caps, strongly decurrent white gills. So far that describes Ampulloclitocybe, but these have forking gills that stain red in age and amyloid spores. (Pseudoclitocybe in the Tricholomatineae has no umbo, no forking, no staining and amyloid spores). Species mentioned: Cantharellula umbonata, oregonensis.   Cantharellula umbonata EU - our ID and WA sequences match EU and CA sequences within 4 bp. East coast sequences also match the EU very well except for a few ambiguous locations. 'Cantharellula' oregonensis OR - In 1942 Singer thought that Murrill's 1913 Clitocybe oregonensis should be called Cantharellula oregonensis, but it doesn't have forking gills and doesn't stain red, so I don't think he was right. Then he himself changed his mind in 1961 and called it Pseudoclitocybe oregonensis. I think that might be right, see the Pseudoclitocybe page for more information. Cantharellula umbonata © Ed Barge and Matthew Koons

Cantharocybe - click to expand A large and stocky, bright yellow, strongly decurrent mushroom often with an off centre stem. It's sort of waxy. Species mentioned: Cantharocybe gruberi.   Cantharocybe gruberi ID -DNA from the type area of Idaho is 6 bp different (plus a couple of ambiguous locations) from multiple sequences from CA and AZ. I wonder if there's more than one species in the west or if the species has variable ITS? One EU sequence is a few bp extra different from all of the above. unsequenced Cantharocybe gruberi © Danny Miller

Chrysomphalina - click to expand Colourful (almost waxy), small mushrooms growing on wood with dry caps. They also have decurrent gills an may be hygrophanous. In fact, this genus is inside the Hygrocybe s.l. clade of waxy caps, but as the wood habitat is unusual and they are not usually thought of as waxies, I deal with them on this page. Species mentioned: Chrysomphalina aurantiaca, grossula, chrysophylla   Chrysomphalina aurantiaca OR - small, completely orange little waxy, with many local sequences. Chrysomphalina grossula EU - yellow-green little waxy. One or two consecutive and equal chunks, four nucleotides long (GCAT) may be missing from our DNA compared to European DNA, but as a single event could drop all those characters, that probably doesn't represent enough of a change to constitute ours being a separate species. Otherwise, there is only 1 or 2bp difference. Chrysomphalina chrysophylla EU - brown capped, golden yellow little waxy. Washington and California DNA matches European DNA, so our local species is the real thing. unsequenced Chrysomphalina aurantiaca and grossula © Kit Scates Barnhart,     unsequenced Chrysomphalina chrysophylla © A and O Ceska

Hygrophorocybe s.l. - click to expand Somewhat waxy looking, otherwise clitocyboid. It wasn't even known what sub-order this genus belonged in until a multi-gene study finally placed it sister to Cuphophyllus, which makes sense. Soon we will know if our species belong in Hygrophorocybe or a new sister genus. Stay tuned.   'Hygrophorocybe PNW01 - one WA collection was white and looked like Hygrophorocybe nivea, but a second collection had a grey-brown pleated cap. A third collection was rich brown and showed a bulbous stem. A fourth was rich brown, which seems to be its typical form, possibly becoming quite pale. 'Hygrophorocybe PNW01  © Buck McAdoo (2 images) and Yi-Min Wang (2 images)

Lichenomphalia and Protolichenomphalia - click to expand One of two known PNW basidiolichen genera (algae usually pair with ascos to form a lichen). The other is Multiclavula, a club. Yellowish tan to brown omphalinoids with grainy or leafy lichen material at the stem base (unlike the very similar Arrhenia, Omphalina, etc.). Our most abundant species may need a new genus.   'Lichenomphalia' ericetorum EU (Lichenomphalia umbellifera misapplied) - dull colours, granular green lichen material at the base. Local sequences match EU sequences within a couple of bp plus a few ambiguous locations. However, this species is not closely related to the type species of Lichenomphalia, described next, and has been placed in its own subgenus, Protolichenomphalia. My ITS-only studies show that it may belong in its own genus, and some other studies have shown that as well "depending on the methods, genes and taxa used" as quoted from another study. A multi-gene study will have to resolve the issue, in the meantime, there is a chance it will have to be renamed to Protolichenomphalia ericetorum. The other interesting thing about this species is that Lichenomphalia umbellifera turned out to refer to 'Marasmius' epiphyllus (and there's a whole story about that on that page), so that name can no longer be used for this species. The proper name is now L. ericetorum, what used to be just a junior synonym. Lichenomphalia cf hudsoniana Nunavut, Canada - more brightly coloured, with leafy green lichen material at the base. It has a somewhat coloured spore print (orange-yellow) and a pubescent stem, growing on tundra in the far north. It's unclear how far down BC it can be found. We need local collections to prove it's here since we only have ENA and Scandinavian DNA. This is the type species of the genus, and gets to stay in Lichenomphalia. Lichenomphalia cf oreades NH (=Clitocybe albimontana NH) -brown to dark brown cap and stem (that is pubescent at first) with paler gills growing from moss and sandy soil. The brown colours make it look exactly like several Arrhenia and one Omphalina, except this species will have granular green lichen material at the stem base. Bigelow reported this from WA, but we need a modern collection to prove it. It is a true Lichenomphalia, as we have the type sequence of L. oreades. One EU sequence of the older Lichenomphalia velutina matches ours (but other sequences with that name from elsewhere do not). The type of L. velutina is a drawing, not a real mushroom, so nobody knows for sure what it is and until that is sorted out, I am not ready to state that we should use that older name. The folks who got the type sequence of L. oreades also got the type sequence of L. grisella, thought to be a newer synonym of L. velutina, and it turns out that L. grisella is not the same species as L. oreades. We need local collections to see if Bigelow really found Lichenomphalia oreades when he discussed Clitocybe albimontana. For now, I'm assuming so. unsequenced Lichenomphalia ericetorum © Steve Trudell

Pseudoarmillariella - click to expand Medium sized thin-fleshed funnel shaped Clitocyboids on conifer logs. Yellowish-tan radially streaky/scaly cap, occasional forked gills that are sometimes quite yellow. Amyloid spores. No other fair-sized white spored mushroom quite like it grows out of logs, even though if you found it growing from the ground you'd be stumped. In fact, I was stumped once when it was on buried wood and the caps were grey (but the gills still showed the yellow).   Pseudoarmillariella ectypoides NY - Our local DNA matches ENA DNA quite well. Pseudoarmillariella ectypoides © Yi-Min Wang and Danny Miller

Spodocybe - click to expand Small somewhat felty grey Clitocyboids. Species mentioned: Spodocybe senilis, trulliformis.   Spodocybe 'trulliformis PNW01' - probably 6-7% different than the EU Spodocybe trulliformis and in need of a new name. This species has distinct felty scales on a grey cap. Spodocybe 'senilis PNW02' - This doesn't seem to be as dark grey nor as felty/scaly as PNW01. It may have been wet, but this species seems to have a streaky, watery-translucent grey cap, easily differentiated from PNW01, and the cap is barely felty if at all. This kind of reminds me of Clitocybe senilis, reported from the PNW as like Spodocybe trulliformis but less scaly on the cap. It should be investigated if reports of that species are this present species. We don't have any sequences of the EU species Clitocybe senilis to compare to. Spodocybe 'trulliformis PNW01' © Danny Miller and Richard Morrison,     S. 'senilis PNW02' © Connor Dooley and Yi-Min Wang

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