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Danny’s DNA Discoveries – Porotheleaceae of the PNW (Marasmiineae)
by Danny Miller

Click here for my Pictorial Key to Clitocybula, Gerronema, Megacollybia and other misc. white spored wood inhabitors.

Introduction

The Porotheleaceae is a good family of genera that belong together, but we don't yet know how they fit into the bigger picture with the Cyphellaceae, which may need to be split and that may affect what happens to the Porotheleaceae. They used to be called the Hydropoid fungi.

Although most genera are gilled, Porotheleum itself is a poroid/cupulate crust. Porotheleum fibriatum EU has been sequenced from the EU and ENA, but we need local sequences to confirm that our species is the real thing too.

abundant common uncommon rare - colour codes match my Pictorial Key and are my opinions and probably reflect my bias of living in W WA. Rare species may be locally common in certain places at certain times.

Click here to download the FASTA data of all my DNA sequences

Summary of Interesting Results

Here are some of the newest, most interesting results of the study:

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Hydropus and Leucoinocybe

Hydropus aff. marginellus EU - our only known local species is a brown "Mycena" growing on wood with brown marginate gill edges and weakly amyloid spores. The genus is separated from Mycena by its different cap cuticle structure (for instance, erect or spherical elements). Lucky, no local Mycenas look exactly like it (none with brown marginate gill edges). Our local species is 4% different from the couple of EU sequences we have so perhaps ours is in need of a new name.

Leucoinocybe sp. - like Hydropus, these are very much like Mycena, with amyloid spores, but perhaps a different cap cuticle. Our local species, only found once in WA, has blue tones like Mycena amicta but presumably doesn't have the peelable cap cuticle and has distant decurrent gills instead of close adnexed gills.

Mycopan was split from Hydropus for M. scabripes and M. pseudotenax, and Mycena quinialtensis belongs in Mycopan as well. They are genetically different than Hydropus and Leucoinocybe but otherwise very difficult to distinguish.

Hydropus aff. marginellus (2 photos) © Yi-Min Wang,     Leucoinocybe sp. (3 photos) © Yi-Min Wang

Gerronema, Megacollybia and Clitocybula

Work still needs to be done to sort these out. See below.

 

Clitocybula familia NY - a tightly clustered greyish-tan small wood inhabiting species. Amyloid spores. One WA sequence matches a few east coast sequences including one Antonin used in his study of the family.

Clitocybula cf abundans NY - more solitary with a depressed, radially streaked greyish cap. Attempts to sequence local collections failed so far, and there are no east coast sequences either. We need local and eastern collections to see if this is our species.

Clitocybula cf lacerata EU - very similar with larger spores. We have the type sequence and other EU sequences that show they have 3 cryptic species over there, probably with variable sized spores, at least 4% in ITS apart from each other. One of their other two species may be C. abundans. We need local sequences to find out if the old report we have of this species from Idaho is true or not, and if so, if it represents the real thing. Perhaps the report of this was actually C. cf. abundans.

Clitocybula familia © NAMA and the Field Museum of Natural History,     C. cf. abundans © Danny Miller

 

Clitocybula oculata OR - described as greyish-brown with a sooty disc, furfuraceous all over and short decurrent gills. But what is it? It is only known from one collection in Oregon  in 1911. The spore size is unique, but we should sequence it to find out if it is indeed placed in the correct genus, especially considering the unanswered questions that follow.

 

Gerronema atrialbum WA - blackish cap and stem contrasting with white gills. A large, very thin-fleshed mushroom with strongly decurrent gills, found on buried wood or debris. We have a half dozen WA sequences that definitely represent this species.

Formerly placed in Clitocybula because of its amyloid spores, but genetics moved it to Gerronema, supposedly with inamyloid spores. But that may not be the final word, since Gerronema is not monophyletic; Trogia and Megacollybia live inside it. Nothing further can be determined until the type species of Gerronema, G. melanomphax from Argentina is sequenced to find out where the true Gerronema are in that complex. Then, perhaps, Gerronema will be split or Megacollybia will be moved to Gerronema.

Gerronema atrialbum © Yi-Min Wang

 

Megacollybia fallax ID - a larger grey-brown wood inhabitor with a radially streaky cap that may split, gill edges that might get eroded in age, and white rhizomorphs at the stem base. Inamyloid spores and these rhizomorphs should separate it from Clitocybula. We have the type sequence. Even though the EU species Megacollybia platyphylla EU was reported here, one study did not find it anywhere on the west coast. If you think you find a different species of Megacollybia here, save it.

This genus, along with Trogia, lives inside Gerronema and therefore does not merit its own genus as currently understood. Either Gerronema will have to be split (see the above discussion) or these may move to Gerronema.

probable Megacollybia fallax © Andrew Parker

 

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