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Danny’s DNA Discoveries – 'Sclerotiniales' of the PNW
by Danny Miller

Introduction

The Leotiomycetes are the inoperculate class of ascos, where the ascii do not have a lid, but instead have a pore at the top that the spores escape from. This is difficult to see without a quality microscope, but the species in the class are usually tiny cup fungi (maximum size <1cm across) growing on wood, as well as the earth tongues (club fungi with a differentiated head). In contrast, the Pezizomycetes contain true truffles, morels and false morels, the larger cup fungi, and tiny cups that do not grow on wood (although there are exceptions). Other classes of ascos exist as well with more cups, truffle-like fungi, and carbonaceous fungi. My ITS tree cannot show the orders holding together, but a 5-gene study and a 15-gene study confirm where I have placed these species.

'Sclerotiniales' is not yet an official order, but probably will become one and be split from the Helotiales, along with the Dermateaceae family. It contains a diverse array of mushrooms.

abundant common uncommon rare - colour codes match my Pictorial Key and are my opinions and probably reflect my bias of living in W WA. Rare species may be locally common in certain places at certain times.

Chlorociboriaceae

Tiny blue cups on wood! The mycelium in the wood can turn the wood itself blue!

 

Chlorociboria aeruginascens EU - the cups have an irregular, wavy margin. Also, the cups grow up to 7mm across, the stems are often off-centre, several cups might arise from the same dark mass, and the interior flesh is blue.

Chlorociboria aeruginosa EU - are usually perfectly round caps. Also, the cups usually only grow to 5mm across, the stems are usually central, the cups rise invididually from the wood, and the interior flesh may have an orange tinge.

Chlorociboria aeruginascens © Mandy Hackney (from CA),     C. aeruginosa © iNaturalist user adisadeh (from CA)

Sclerotiniaceae

This is a family of parasites. It would be nice to keep thinking of it as its own family, since these parasites clade nicely together here, but since this family lives paraphyletic inside the Rutstroemiaceae family, that family would have to be split up awkwardly into four or more families if you don't allow a family to live inside another family. Fortunately, I do, so I'm not going to worry about it.

The following species clade so close together that it seems they should all be one genus. That means that Botrytis, Ciboria, Sclerotinia, Ciborina, Monilinia, Myriosclerotinia, Ovulina, and Sclerencoelia may need to be absorbed into one genus. Botrytis is the oldest asexual (anamorph) genus. Ciboria and Sclerotinia are tied for the oldest sexual (teleomorph) genus. Since the family is the Sclerotiniaceae, perhaps Sclerotinia is an appropriate genus for them all to be absorbed into. Or perhaps they will stay separate genera, setting a record for how little ITS difference can constitute distinct genera.

Botrytis cinerea EU - an asexual mold that causes noble rot of grapes that can make interesting wines. We have worldwide sequences.

Ciboria amentacea EU? - what I think are type area sequences show us what this is. We need local collections.

Ciboria caucus EU - the type species of the genus that shows us what true Ciboria are. We need local collections.

Ciboria gordonii BC - no DNA yet, so I don't know the true genus.

Ciboria seminicola OR - no DNA yet, so I don't know the true genus.

Ciborinia whetzelii NY - we don't have a sequence of the type species to know if this is the correct genus. We need local collections.

Monilinia demissa ENA? - no DNA, so I don't know the true genus. We need local collections.

Monilinia fructicola PN - type of genus. We need local collections.

Monilinia gregaria WA - no DNA yet, so I don't know the true genus.

Monilinia laxa EU - an OR and BC sequence match worldwide sequences. We need local photos.

Myriosclerotinia caricis-ampullaceae EU - one OR sequence matches a sequence that might be from the EU. We need local photos.

Myriosclerotinia curreyana UK - I think we have a type area sequence of this. We need local collections.

Myriosclerotinia duriaeana EU - I think we have a type area sequence of this. We need local collections.

Ovulinia perplexa WA - no DNA, so I can't confirm the genus, but we have sequence of the type species. We need local collections.

Sclerencoelia pruinosa CO (=Encoelia pruinosa) - doesn't clade with the type species, may need a new genus. We need local collections.

Sclerotina juncigena WA -no DNA yet to confirm the genus. We need local collections.

 

These seem justified in being outside of Sclerotinia s.l., but are still inside the Sclerotiniaceae.

Pseudociboria umbrina WA - monotypic, no DNA, so I can't confirm its placement. We need local collections.

Mycopappus alni WA - one NBC sequence shows us what this is. We need local photographs.

Valdensinia heterodoxa EU - we need local collections.

Rutstroemiaceae

The Sclerotiniaceae is paraphyletic inside this family. Here is everything from that big family that is not in Sclerotiniaceae s.s.

 

'Lanzia' 'luteovirescens PNW01' - olive yellow-green cups on a long stem growing from leaves. Our sequences are 3% different in ITS from EU type area sequences, so our species may need a new name. Also, it's unclear what genus this belongs in. Some say Lanzia is a genus in the Helotiaceae. Some say it's in a different place in this family than this species. Either way, it would need a new genus.

Rutstroemia sydowiana EU - tiny brown discs on oak leaves with a short stem. There are 2 clades of EU sequences differing by about 1% in ITS. An OR sequence matches one of them. I'm assuming for now that they are both the same species and that we don't need a new name for ours.

Lambertella pruni OR - brown discs on sweet cherry fruits and mummified fruits. We have type area sequences but need photographs.

'Cenangium' cf acuum NY - irregularly shaped reddish-brown spheres/cups on pine and juniper needles. We have EU sequences that differ amongst themselves by 1% or so in ITS, but no type area sequences nor local sequences. Ekanayaka thought this represented the genus, but it may be in a different genus than the type species of the genus, C. ferruginosum, discussed next. Johnston's 15-gene tree showed that this species likely needs a new genus and belongs in the greater Rutstroemiaceae/Sclerotiniaceae family.

'Moellerodiscus' tenuistipes EU - a long stemmed cup with a brown upper surface and grey lower surface on alder leaves. There is no DNA available, so I can't confirm the genus or family, but several concepts of this genus both show it in the greater Rutstroemiaceae/Sclerotiniaceae family, so that's probably the family that this belongs in. It may well need a new genus. We need collections.

Clarireedia calopus EU - a typical long stemmed brown cup. This species was not known from the PNW until sequenced from WA.

Clarireedia maritima EU - a typical long stemmed brown cup. This species was not known from the PNW until sequenced from OR.

'Stromatinia' gladioli ENA? - has been reported from the PNW. We have an old sequence from the person who described it, and it belongs in Clarireedia, not Stromatinia (which is one of the general very close to Sclerotinia). We need local collections.

'Sclerotinia' veratri CO - one CA sequence suggests it belongs in or near Clarireedia.

'Monilinia' oxycocci EU - a few sequences show us this needs a new genus. It has been reported from here, but we have no local DNA yet.

'Monilinia' urnula Russia - 2 EU? sequences show us this needs to move to the same genus as M. oxycocci. It has been reported from here, but we have no local DNA yet.

Sclerotiniaceae PNW01 (Ciboria "rufofusca-CA01") (Ciboria rufofusca EU misapplied) - a long stemmed brown cup often on Douglas fir cones. CA sequences of CA01 don't quite match an EU sequence of C. rufofusca, so they gave it a code. Our PNW sequences don't quite match either, so perhaps ours needs its own code. These need a new genus, likely in the Cenangiaceae or the Rutstroemiaceae.

Sclerotiniaceae PNW02 - a short stemmed brown cup on conifer debris. My ITS tree shows this more likely belongs in the Cenangiaceae or the Rutstroemiaceae, and not in Sclerotiniaceae s.s.

'Lanzia' 'luteovirescens PNW01' © Kitty Lundeen,     Rutstroemia sydowiana © Carolyn Delevich,     Clarireedia calopus © Jordan Gates,     Clarireedia maritima © Leah Bendlin

Sclerotiniaceae PNW01 © Yi-Min Wang,     Sclerotiniaceae PNW02 © iNaturalist user steller_viscera

 

Cenangiaceae

Although Ekanayaka's 5 gene study keeps Hemiphacidiaceae as a separate family, Johnston's 15-gene study shows both families should be combined into the Cenangiaceae, so for simplicity, that's what I am following.

Cenagnium cf ferruginosum EU - yellowish brown cup-shaped fruitbodies with a dark reddish brown powdery coating on the exterior on conifer branches. This is the type species of the genus. We have a bunch of type area sequences but need local collections.

Chlorencoelia cf versiformis EU - small olive yellow-green cups (<1cm across) on decayed wood. We have EU and ENA sequences of this. ENA also has C. torta PN as well. We need local sequences to see which species we have.

Encoelia cf furfuracea EU - brown cups coated in a mealy powder than can be rubbed off, growing on alder.

Heyderia PNW01 - a brown earth tongue with a pinkish-buff head and a brown stem. Heyderia abietis UK is the species reported from here, but our DNA is almost 5% different in ITS and so our species probably needs its own name.

Heyderia PNW02 - one OR sequence was almost 3% different, this sequence should be looked for again to verify it.

Velutarina rufo-olivacea EU - the type species. We have EU and ENA sequences that show us what this is. We need local collections.

Heyderia PNW01 © Jonathan Frank,     Heyderia PNW02 © Heather Dawson

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